Journal: Methods in Molecular Biology

Article Title: Pain Research

doi: 10.1007/978-1-61779-561-9

Figure Lengend Snippet: Fig. 1. Experimental design used for the induction of acute and chronic pancreatitis models in rats. The acute model is induced with a tail vein injection of DBTC and nociceptive hypersensitivity peaks at 1 week. Hypersensitivity develops within 3 weeks in Fisher rats fed a high fat and alcohol diet and persists for 7 weeks. In both models, referred pain is present on the rat’s abdomen and in a cutaneous band extending dorsally to the shoulder and neck (hatched region).

Article Snippet: High fat commercial diet with 28% fat (LD101A with LD104, TestDiet, Richmond, IN).

Techniques: Injection

Journal: Methods in Molecular Biology

Article Title: Pain Research

doi: 10.1007/978-1-61779-561-9

Figure Lengend Snippet: Fig. 2. Photomicrographs of immunohistochemical staining of regulated on activation, normal T cells expressed and secreted (RANTES) in the pancreas ( a ) from a naïve rat, ( b ) from a rat 1 week after dibutyltin dichloride (DBTC)-induced pancreatitis and ( c ) from an animal 10 weeks after induction of pancreatitis with a diet of high fat and alcohol (6%). RANTES is a chemokine attracting infl ammatory cells to the infl amed pancreas. The small particles evident in the widened ducts ( spaces ) surrounding the pancreatic exocrine cells are the invading infl ammatory cells (leukocytes) Scale Bar: 50 um.

Article Snippet: High fat commercial diet with 28% fat (LD101A with LD104, TestDiet, Richmond, IN).

Techniques: Immunohistochemical staining, Staining, Activation Assay

Journal: Methods in Molecular Biology

Article Title: Pain Research

doi: 10.1007/978-1-61779-561-9

Figure Lengend Snippet: Fig. 5. The response times to hotplate are shown for control rats and rats fed the high fat and alcohol diet for 4 weeks. Responses times that are signifi cantly less for the animals with pancreatitis for 3–6 weeks indicate hypersensitivity of the animals fed the high fat and alcohol diet.

Article Snippet: High fat commercial diet with 28% fat (LD101A with LD104, TestDiet, Richmond, IN).

Techniques: Control